An easy way to tune the bass guitar by itself is by using harmonics. Harmonics sound clearer and higher than normal bass notes and therefore their pitch is easier to distinguish. Remember to place your left hand finger exactly over the fret, only lightly touching the string. Pick the note with your right hand close to the bridge to get the clearest, brightest sound. (Let the harmonics ring).
The harmonics used for tuning are located on the 5th and 7th fret.
The 7th fret on the G string =the 5th fret on the D string.
The 7th fret on the D string =the 5th fret on the A string.
The 7th fret on the A string =the 5th fret on the E string.
The notes on the fretboard diagram which are linked together have the same pitch.
When tuning with harmonics always start on the highest string (G string) and adjust the other strings to it. Play the 7th fret on the G string, let it ring and while it is still sounding, play the 5th fret on the next string down (D string). Let both strings ring at the same time. If they are not perfectly in tune you will hear a pulsing (beating) sound. The slower the pulse the closer you are in tune. The aim is to turn the tuning peg of the 2nd string while both strings sound until the "wave" disappears and both strings sound exactly the same.
Once the first two strings are in tune repeat the same process starting on the 7th fret on the D string and adjust the note on the 5th fret on the A string, then do the same again; play the 7th fret on the A string and adjust the 5th fret on the E string until they match.